You multiply the original concentration by the dilution factors for each dilution. Explanation: A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step. In serial dilutions, you multiply the dilution factors for each step. Example 1 What is the dilution factor if you add 0. Example 2 If you did the above dilution four times, what would be the final dilution factor?
Solution 2 Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume. Related questions How do you calculate concentration from absorbance? How do you calculate concentration from titration? How do you calculate dilution factor?
How to calculate concentration of solution when it's diluted? Find out more article Last updated on December 8th, ,. Report Feedback Login Please. Steps of A ten-fold dilution. Steps of Ten-fold serial dilutions. Steps of A two-fold dilution. Steps of Two-fold serial dilutions. Log Dilutions. Decimal Numbers vs Scientific Notation.
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Cancel Share. In an experiment involving concentration curves, you can use a serial dilution to create a series of solutions with dilutions of 1, , , , Method 2. Calculate the final dilution ratio in a serial dilution. The total dilution ratio can be determined by multiplying the dilution factor of each step leading up to the final step. The concentration of your substance is now 10, times less than the original undiluted solution. Determine the concentration of the solution following dilution.
To determine the final concentration of your solution following serial dilution you will need to know your starting concentration.
Confirm that all units match. When performing any calculation, you want to make sure that your units always match at the end. If your starting concentration is parts per million ppm , then your final concentration must be ppm. Not Helpful 3 Helpful During serial dilution, why do I need to change the tips and mix well before going to the next dilution?
Change the tips in order to get an accurate dilution. Mix well in order to ensure the solution has equal amounts throughout. Not Helpful 8 Helpful The molecules are dispersed in water and are farther apart, making the concentration weaker.
Not Helpful 2 Helpful 8. If there was no visible bacterial colony formed on my plate, what can I conclude? Different things can be concluded. The incubation temperature of your plate might have been too high or too low. The media that was used could also be a problem; some bacteria cannot grow in certain media while others can. In addition, the bacteria might need a longer time than usual to show growth. Any of these could be a possibility, which means more tests need to be done to completely understand and address the issue and come up with a conclusion.
Not Helpful 2 Helpful 4. How can I set up a series of tubes with a dilution such that I end up with 3mL of solution in each tube at the end of the procedure? If you're using 4 tubes, altogether, after dilution, that's 12mL. It's the 3mL times the number of tubes, which is 4. One quarter of 12mL is 3mL. This means you should have 3mL in each tube if you divide the total amount into the 4 tubes. Not Helpful 27 Helpful 8. If I had 2 ml of a substance and then added 4 ml of a dilution, what would my ratio be?
Not Helpful 1 Helpful 7. Include your email address to get a message when this question is answered. By using this service, some information may be shared with YouTube. You Might Also Like How to. How to. About This Article. Co-authored by:. Co-authors: Updated: November 30,
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